FVIII inhibitors display FV‐neutralizing activity in the prothrombin time assay
نویسندگان
چکیده
منابع مشابه
Comparative measurement of FVIII inhibitors in hemophilia A patients using ELISA and the Bethesda assay.
Factor VIII inhibitors are produced during or after coagulation factor VIII (FVIII) therapy in hemophilia A patients. These inhibitors are usually detected by a modified Bethesda assay or an enzyme-linked immunosorbent assay (ELISA). In this study, we used the Bethesda assay to determine the incidence of FVIII inhibitors in 75 fresh plasma samples obtained from 50 hemophilia A patients, and the...
متن کاملFVIII inhibitors: pathogenesis and avoidance.
The pathogenesis of inhibitory antibodies has been the focus of major scientific interest over the last decades, and several studies on underlying immune mechanisms and risk factors for formation of these antibodies have been performed with the aim of improving the ability to both predict and prevent their appearance. It seems clear that the decisive factors for the immune response to the defic...
متن کاملNative plasma-derived FVIII/VWF complex has lower sensitivity to FVIII inhibitors than the combination of isolated FVIII and VWF proteins. Impact on Bethesda assay titration of FVIII inhibitors
Sensitivity to FVIII inhibitors of the native plasma-derived (pd) FVIII/VWF complex vs. the complexes formed after exogenous FVIII infusion in the haemophilic patient has not been thoroughly studied. The role of VWF in the interaction of FVIII with inhibitors was studied in vitro using different combinations of VWF and FVIII concentrates. Normal plasma, pdFVIII/VWF and isolated FVIII (recombina...
متن کاملA Novel Approach to Treating FVIII Inhibitors: In Vitro Results for Combination Therapy with Human Prothrombin Complex Concentrate and Plasma-Derived FVIII/VWF Complex
Coagulation factor VIII (FVIII) inhibitors are the most severe complications of haemophilia A treatment. We studied the effect of a non-activated prothrombin concentrate (PCC) combined with FVIII in vitro. FVIII antibodies minimised the in vitro thrombin generation of standard human plasma and prolonged the lag phase at a residual FVIII activity of ≤ 0.01 IU/ml. Time to clotting of FVIII-inhibi...
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Background: Psoriasis is a common chronic and recurrent inflammatory skin disorder related to the immune system and characterized by the rapid growth of skin cells. To date, no study has been conducted on the role of the prothrombin time index in determining the disease activity and the prognosis of these patients. Recognizing the factors influencing the prognosis of this disease can be helpf...
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ژورنال
عنوان ژورنال: Journal of Thrombosis and Haemostasis
سال: 2021
ISSN: 1538-7933,1538-7836
DOI: 10.1111/jth.15355